通过谷歌学术搜索发现，自2010年以来，就有大量科研人员使用Simgen产品进行科学研究并发表学术论文。不仅研究论文数量巨大，而且有一批高质量的注明使用Simgen产品的研究论文发表在Cell、Nature、Cell Stem Cell、Immunity、Developmental Cell、Cell Reports、Nature Medicine、Nature Immunology、Nature Chemical Biology、Nature Cell Biology、Nature Communications、PNAS、Blood、Gastroenterology、Nature Protocols、Plant Cell、Hepatology等高水平期刊上。
所有Simgen员工为此感到欢欣和鼓舞，感到骄傲和自豪！广大用户大量标注Simgen的研究论文的发表是对所有Simgen最大的支持和鼓励，最真切的认可和回报！
作为Simgen人，我们会再接再厉，不断开拓进取，为广大用户提供更多高质量的产品和服务，继续踏踏实实地为中国的生命科学事业添砖加瓦，以答谢广大用户对我们的支持和鼓励，对我们的认可和回报!
Simgen将永远和您一起为中国的生命科学事业的腾飞贡献自己的一份力量！！！
LJ Deng, WX Ouyang, R Liu, M Deng, JW Qiu… - Liver …, 2021 - Wiley Online Library
… Peripheral blood of 2 mL was collected from each subject, and the genomic DNA was
extracted using a DNA extraction kit (Simgen, China) according to the manufacturer’s instructions. …
YH Zheng, XB Cai, LQ Xia, FY Zhou, XR Wen… - Molecular …, 2021 - ncbi.nlm.nih.gov
… in peripheral blood by using the Blood DNA Mini Kit (Simgen, Hangzhou, China) [30]. …
The genomic DNA was sheared into 100 base pairs via the Exome Enrichment V5 Kit (Agilent …
J Bai, Q Ma, Y Lan, Y Chen, S Ma, J Li, C Liu… - Frontiers in Cell and …, 2021 - frontiersin.org
… From each subject, 400 μL whole blood was treated according to the SimGEN Blood DNA
… The human ELISA kit was produced by Abcam. A standard sample or plasma, horseradish …
B Chen, X Yu, X Zhang, H Yang, Y Cui… - Journal of …, 2022 - hindawi.com
… ese tubes were preserved at ?80C before genomic DNA was extracted with a Simgen DNA
Blood Mini Kit (Simgen, Hangzhou, China) according to the manufacturer’s instructions. All …
Y Xiang, B Liang, X Zhang, X Qiu… - Clinical …, 2022 - clinicalepigeneticsjournal …
… Genomic DNA was extracted using the phenol/chloroform method or a commercial DNA
extraction kit (3001050, Simgen, Hangzhou, China) based on the sample size and was …
6. Novel SOX2 mutation in autosomal dominant cataract-microcornea syndrome
ZB Lin, J Li, L Ye, HS Sun, A Yu, SH Chen, FF Li - BMC ophthalmology, 2022 - Springer
… A conventional DNA extraction kit (Simgen, Hangzhou, China) was used for genomic
DNA extraction. In addition, blood samples from another 200 healthy controls without of eye …
1.The complete mitochondrial genome of the common strain black carp (Cyprinus carpio?var. baisenensis)
LJ Deng, WX Ouyang, R Liu, M Deng, JW Qiu… - Liver …, 2021 - Wiley Online Library
… Peripheral blood of 2 mL was collected from each subject, and the genomic DNA was
extracted using a DNA extraction kit (Simgen, China) according to the manufacturer’s instructions. …
T Xiong, Y Zeng, W Wang, P Li, Y Gai, C Jiao, Z Zhu… - Journal of Fungi, 2021 - mdpi.com
… The scraped mycelia were lyophilized, and then the DNA was extracted using a SIMGEN
Fungi Kit (Simgen, Hangzhou, China) according to the manufacturer’s protocol. The extracted
Y Zeng, T Xiong, B Liu, E Carstens, X Chen… - …, 2021 - Am Phytopath Society
… The mycelia were scraped from the plates and lyophilized before DNA extraction with a
SIMGEN Fungi Kit (Simgen, Hangzhou, China) according to the manufacturer’s protocol. The …
W Zhu, M Yu, R Xu, K Bi, S Yu, C Xiong… - Environmental …, 2022 - Wiley Online Library
… Total RNA of fungi and plant samples were isolated using RN03-RNApure Kit (Aidlab, …
cinerea strains was isolated using Fungal Genomic DNA Purification Kit (Simgen, Hangzhou…
H Weng, Y Liu, I Captoline, X Li, D Ye, R Wu - Computers and Electronics in …, 2021 - Elsevier
… it. The DNA was extracted using a Plant DNA kit (Simgen, Hangzhou, China). TaqMan
probes and primer pairs were synthesized according to the reported study by a
biotechnology company (Tsingke, Hangzhou, China). The …
W Yang, J Ren, W Liu, D Liu, K Xie, F Zhang… - Horticultural Plant …, 2022 - Elsevier
… The genomic DNA was isolated from citrus protoplasts and the mutant shoots using plant
DNA Kit (SIMGEN, China). The genomic fragments containing the target sites were amplified by …
3.Microbial diversity on the surface of historical monuments in Lingyan Temple, Jinan, China
T Li, Y Cai, Q Ma - Microbial Ecology, 2022 - Springer
… Total genomic DNA was extracted with a Plant DNA Isolation Kit (Simgen, Hangzhou, China)
according to the manufacturer’s protocols. The DNA was subsequently used as a template …
4.The chloroplast genome of Salix floderusii and characterization of chloroplast regulatory elements.
W Ren, Z Jiang, M Zhang, L Kong, H Zhang… - Frontiers in plant …, 2022 - europepmc.org
… Total genomic DNA was extracted using the Plant DNA Extraction Kit (SIMGEN, Hangzhou,
China) according to the manufacturer’s instructions. DNA purity and integrity were verified via …
Y Cai, H Cao, F Wang, Y Zhang, P Kapranov - Nature communications, 2022 - nature.com
… Genomic DNA from sperm cells was extracted using a Sperm DNA Purification Kit (Simgen,
4202050) following the manufacturer’s instructions. All DNA samples were eluted in …
J Wu, Y Yin, M Qin, K Li, F Liu, X Zhou… - Shock (Augusta …, 2021 - ncbi.nlm.nih.gov
… gut tissues using TRIzol reagent (Simgen, China). The same amount of total RNA was
reverse transcribed to cDNA using the ReverTra Ace qPCR RT kit (Simgen, China). Quantitative …
L Liang, Q Xiong, J Kong, C Tian, L Miao… - Biomedicine & …, 2021 - Elsevier
… isolated using TRIzol reagent (Simgen, Hangzhou, China) … using a Whole Protein
Extraction kit (KeyGen BioTech, China) … an enhanced chemiluminescence detection kit (biosharp, …
Z Yu, YY Zhao, N Jiang, AZ Zhang, MY Li - Fish & Shellfish Immunology, 2021 - Elsevier
… kits (Shanghai Biotechnology Co., Ltd., Shanghai, China). All measurement steps were
performed according to the relevant kit … extracted using TRIzol Reagent (simgen) according to the …、
4.A transcription factor DAF-5 functions in Haemonchus contortus development
W Di, F Li, L He, C Wang, C Zhou… - Parasites & …, 2021 - parasitesandvectors.biomedcentral …
… contortus using TRIzol reagent (Simgen, China). All RNA samples were treated with
DNase I to remove genomic DNA (gDNA), and then their integrity and yields were verified by …
Y Jiang, Z Peng, Q Man, S Wang… - Biology of sex …, 2022 - bsd.biomedcentral.com
… Total RNA was extracted using TRIzol reagent (Simgen) and cDNA was prepared using
PrimeScript RT reagent Kit with gDNA Eraser (Takara, RR047A). Analysis of mRNA expression …
6.中国南方一罕见迟发性脊椎骨骺发育不良大家系的表型和基因型的相关性研究
黄颖， 谢杰， 郑诗瑶， 唐佳， 郭奕斌 - 中山大学学报(医学科学版), 2022 - researchgate.net
...1.2.3 逆转录 PCR（RT-PCR）使 用 Trizol 试 剂（Simgen 试剂盒），从受试者新鲜外周血白细胞中提
取总 RNA，在 20 μL 体积内反向转录 1 000 ng RNA（Fermantas 试剂盒）...
A Ayaz, H Huang, M Zheng, W Zaman, D Li… - International journal of …, 2021 - mdpi.com
… Triplicate biological samples of either soybean or Arabidopsis were used for the extraction
of total RNA using the Trizol total RNA extraction kit (SIMGEN, Beijing, China) according to …
2.A novel homozygous mutation in the PADI6 gene causes early embryo arrest
X Wang, H Zhu, Y He, J Zeng, J Zhao, Q Xia, L Wu… - Reproductive health, 2022 - Springer
… after transfection using the Total RNA Purification Kit (SimGen). Reverse transcription and
amplification were performed using the Evo M-MLV RT Mix Kit (Accurate Biotechnology), and …
Y Tian, B Wu, L Peng, J Wang, M Shen - Frontiers in Immunology, 2022 - ncbi.nlm.nih.gov
… Total RNA was extracted from frozen blood using an RNA isolation kit (Simgen, 5201005).
RNA was reverse transcribed using a 1st Strand cDNA Synthesis Kit (Takara, 6110). The …
2.Role of NINJ1 in Gout Flare and Potential as a Drug Target
H Zhang, J Gao, W Fang, Y Tang, X Fang… - Journal of …, 2022 - dovepress.com
… Total RNA was isolated from whole blood using a whole Blood RNA isolation kit (Simgen),
total RNA was isolated from cultured cells using Hipure total RNA mini kit (Magen). Isolated …
Y Yin, X Liu, Q Tian, Y Fan, Z Ye, T Meng, G Wei…- medRxiv, 2022 - medrxiv.org
… Total RNA was isolated from 2.5ml blood using total RNA isolation kit from SIMGEN. Isolated total RNA and remaining blood sample were subjected to mRNA sequencing and whole-…
Q Zhang,S Li, P Lei, Z Li, F Chen, Q Chen, Y Wang… - Virologica Sinica, 2021 - Springer
… Three micrograms of RNA was reverse transcribed into cDNA using Toyobo RTkits with a …in each supernatant, viral nucleic acid isolation kits (Simgen, China) were used to extract the …
X Liu, J Liang, Y Yu,X Han, L Yu, F Chen… - Journal of Medicinal …, 2022 - ACS Publications… Supernatant virion RNA was extracted with the Viral Nucleic Acid Purification Kit (Simgen) according to the manufacturer’s instructions. Viral RNA was reverse transcribed into cDNA by …
J Feng, D He, T Chen, W Cai, Y Chen, Y Xiong, Q Wu… - 2021 - researchsquare.com
… The contents in the colon of the piglets was aseptically collected, and the total DNA of the colonic contents was extracted using a DNA Kit (SimGEN, Hangzhou, China) according to the …
R Zhen, C Liu, C Wei, Y Luo, X Hu, G Liu…- Journal of Applied …, 2022 - Wiley Online Library
… The feces of the piglets were aseptically collected, and the total DNA of the feces 111 was extracted using a DNA Kit (SimGEN, Hangzhou, China) according to the 112 instructions …
3.Effects of Niacin on Resistance to Enterotoxigenic Escherichia coli Infection in Weaned Piglets
R Zhen, J Feng, D He, Y Chen, T Chen, W Cai… - Frontiers in …, 2022 - ncbi.nlm.nih.gov
… The contents in the colon of the piglets were aseptically collected, and the total DNA of the
colonic contents was extracted using a DNA Kit (SimGEN, Hangzhou, China) according to the …
1.Genome-wide characterization of C2H2 zinc-finger gene family provides insight into the mechanisms and evolution of the dehydration–rehydration responses in Physcomitrium and Arabidopsis
Xuan Li, Cao, Jialing Li, Qinqin Niu, Yuanping Mo and L Xiao - researchgate.net
cDNA was generated using HiScriptII 1st Strand cDNA Synthesis Kit (Vazyme, China). The gene… study were extracted and purified usin the Simgen Plasmid Extract Kit (Simgen, China). …
Y Yao, S Deng, F Zhu- Genes, 2022 - mdpi.com
… Subsequently, products were excised from the gel, purified with a commercial kit (DNA Gel ExtractionKit, 2001250, SIMGEN, Hangzhou, China) and sequenced by Sanger sequencing. …
2.Clinical Performance of Nanopore Targeted Sequencing for Diagnosing Infectious Diseases
Y Fu, Q Chen, M Xiong, J Zhao, S Shen…- Microbiology …, 2022 - Am Soc MicrobiolL Mi, D Ma, S Lv, S Xu, B Zhong, T Peng, D Liu, Y Liu- Genes, 2022 - mdpi.com
… using the first strand cDNA synthesis kit (Simgen) according to the … using a SYBR Green PCR Mix kit (Simgen) with 10-μL … extracted using miRNA purification kits (Simgen, Hangzhou, …
2.Polymeric immunoglobulin receptor in dongtingking crucian carp (Carassius auratus indigentiaus): Molecular characterization and expression analysis in response to?
H Xia, P Yang, Z Chen, Y Zhang, L Liu… - Aquaculture …, 2022 - Wiley Online Library …
...The total RNA was extracted using the animal tissue total RNA extraction kit (Simgen). RNA … Reverse transcription was performed usinga reverse transcription kit (Simgen)and the …
1.Integrated transcriptome sequencing and RNA interference reveals molecular changes in?Diaphorina citri?after exposure to validamycin
HZ Yu, YX Xie, J Wang, Y Wang, YM Du… - Insect …, 2021 - Wiley Online Library… Total RNA was isolated from the control group (CK), V12, V24, and V36 using an animal tissue total RNA kit (Simgen, Hangzhou, China) following the manufacturer's protocol. In this …
2.Silencing of?Glycogen Synthase Kinase 3?Significantly Inhibits Chitin and Fatty Acid Metabolism in Asian Citrus Psyllid,?Diaphorina citri
JB Zhang, ZJ Lu, HZ Yu- International journal of molecular sciences, 2022 - mdpi.com
Thetotal RNA was isolated from D. citri in different tissues (midgut, leg, head, wing,integumentand fat body) and at different developmental stages (egg, 1st-, 2nd-, 3rd-,4th-and 5th-instar nymphs and adults) using the animal tissue total RNA kit (Simgen).
3.Polymeric immunoglobulin receptor in dongtingking crucian carp (Carassius auratus indigentiaus): Molecular characterization and expression analysis in response to?
H Xia, P Yang, Z Chen, Y Zhang, L Liu… - Aquaculture …, 2022 - Wiley Online Library
… The total RNA was extracted using the animal tissue total RNA extraction kit (Simgen). RNA… Reverse transcription was performed using a reverse transcription kit (Simgen)and the …
P Wang, X Jia, X Xiao, S Li, Y Long, M Liu…- Frontiers in Cell and …, 2021 - frontiersin.org… (HeLa) according to the manufacturer’s instruction (Rapid Plasmid Mini Kit, SIMGEN). The transfected cells were cultured for 48 h and then collected for transcript expression analysis. …
2.The identification of a novel splicing mutation in the DMD gene of a Chinese family
W Liu, X Shi, Y Li, F Qiao, Y Wu- Clinical Case Reports, 2021 - Wiley Online Library
… The positive colonies were used for extracting plasmid by Rapid Mini Plasmid Kit (Simgen),and plasmid was subsequently sequenced to confirm the inserts. The confirmed plasmids …
3.A Novel Mutation c. 3392G> T of COL2A1 Causes Spondyloepiphyseal Dysplasia Congenital by Affecting Pre-mRNA Splicing
L Fan, L Ji, Y Xu, G Shen, K Tang, Z Li, S Ye…- Frontiers in …, 2022 - ncbi.nlm.nih.gov… The wild-type and mutated amplified products were purified by a gel extractionkit and then … After plasmid amplification in DH5α competent cells and plasmid extraction (SIMGEN), the …
L Mi, D Ma, S Lv, S Xu, B Zhong, T Peng, D Liu, Y Liu- Genes, 2022 - mdpi.com… using the first strand cDNA synthesis kit (Simgen) according to the … using a SYBR Green PCR Mixkit (Simgen) with 10-μL … extracted using miRNA purification kits (Simgen, Hangzhou, …
2.Conjugate of ibrutinib with a TLR7 agonist suppresses melanoma progression and enhances antitumor immunity
S Ren, X Wang, G Jin- International journal of biological sciences, 2022 - ncbi.nlm.nih.gov
… cDNA synthesis and real-time PCR were performed using a cDNA synthesis master mixkit and 2×SYBR Green PCR Mix (Simgen, Hangzhou, China). The following primers were used: c…
3.甜橙SPL 基因家族的鉴定及其在成花诱导中的表达分析
杨杰， 陈蓉， 胡文娟， 吴巧玲， 陈健美…- 西北植物学报, 2022 -
… 使用植物总RNA 提取试剂盒(Simgen,中国杭州) 提取样品总RNA,使用反转录试剂盒(Simgen,中国杭州)反转录合成cDNA.利用2×SYBR Green PCR Mix(Simgen,中国杭州)在Light-Cycler480 …
1.Crosstalk between the gut microbiome and clinical response in locally advanced thoracic esophageal squamous cell carcinoma during neoadjuvant?…
L Xu, Y Qi, Y Jiang, Y Ji, Q Zhao, J Wu…- Annals of …, 2022 - ncbi.nlm.nih.gov
… The samples were dissolved in into fecal DNA preservation fluid (Simgen, 4103100,Hangzhou, China), transported to the laboratory, and stored at ?8 ℃ for subsequent processing. …
1.Trigeminal Nerve Derived-Substance P Regulates Biological Characteristics of Limbal Stem Cells by Modulating the PI3K-AKT Signaling Pathway
P Xu, X Lin, X Dong, Y Liu, Z Wang, S Wang - Available at SSRN 4237970 - papers.ssrn.com
… Limbal tissues were quickly dissected and placed in RNA later reagent (Simgen, Hangzhou,China)for RNA isolation. Each sample contained three to five independent repeated mice …
·常温保存1周，37℃保存1天，4℃至少保存1个月，组织4℃浸泡过夜后-20℃或-80℃可长期保存。·反复冻融：冻存于-20℃或-80℃的组织可反复冻融20次而不影响RNA提取的质量。 查看产品
·15分钟内即可完成血液总DNA的制备·无须事先分离去除红细胞及蛋白酶K消化步骤，可室温运输储存·彻底清除血样中的PCR抑制物，可使用多至1/2反应体系体积的模板进行扩增 查看产品
·样本通用性强：植物、真菌、粪便、细菌、动物组织、血液均可适用·样本取样范围大：50~200 mg均可适用，特别适合从无法彻底溶解的生物样本中提取总DNA·操作步骤简洁、快速，无需蛋白酶K消化及添加乙醇步骤，30-40分钟内即可完成植物总DNA的制备·特殊设计的溶解液有效应对植物中的多糖衍生物的干扰·特别优化配置的核酸纯化柱仅高效吸附大片 查看产品
·经典蛋白酶K消化步骤，适合从各种不同来源的动物组织（包括鼠尾、鼠耳、福尔马林固定组织等）中分离纯化DNA·可从体液样本（包括抗凝全血、唾液、培养细胞悬液等）分离纯化DNA·可从昆虫、革兰氏阴性菌或革兰氏阳性菌中分离纯化DNA· 无须酚氯仿抽提及异丙醇沉淀步骤 查看产品
· 20-40分钟内即可完成体液中总核酸的制备，同步分离纯化病毒DNA或病毒RNA· 较传统的煮沸法提取病毒DNA相比，检测灵敏度增加10-50倍以上. 较传统Trizol法提取病毒RNA相比，检测灵敏度增加5-10倍以上· 添加的Carrier RNA有效提高病毒的检测敏感性（稳定检测到体液中浓度为100 copies/ml的病毒）· 无须酚氯仿抽提及异丙醇沉淀步骤· 所获的核酸不含杂质及抑制物 查看产品
·40-50分钟内即可完成粪便总DNA的分离与纯化·可同步分离纯化人或动物肠道脱落细胞的DNA、细菌DNA、病毒DNA及寄生虫DNA·无须酚氯仿抽提及异丙醇沉淀步骤·柱纯化技术彻底去除粪便中PCR抑制物 查看产品
· 20-40分钟内即可完成精液总DNA的制备。· 可从新鲜或者是冷冻的精液中分离纯化总DNA。· 直接从200 μl精液样本中分离纯化DNA，无需事先分离精子细胞。· 核酸纯化住可最大吸附20 μg总DNA。· 所获得DNA长度在200bp-50kb之间，主要的长度在30kb左右。 查看产品
·添加的Carrier RNA能有效提高DNA的回收效率·适用于各种不同来源的含微量DNA的样本·无须酚氯仿抽提及异丙醇沉淀步骤·25 μl微量洗脱体积洗脱核酸·可用于指纹印迹和亲子鉴定等基因分型实验 查看产品
·20-40分钟内即可完成全血中总RNA的分离与纯化·无须事先裂解去除红细胞·全血样品可用裂解液裂解后﹣20℃保存一周后再使用·所获的RNA不含杂质及抑制物，模板可多加至反应体系的1/2体积进行扩增 查看产品
·20-40分钟内即可完成总RNA的分离与纯化·经典酸性酚萃取法与柱纯化法的完美结合·无需氯仿，配置的Buffer EX可完美替代氯仿·样本：动物组织、细胞、植物组织、血液等·应用：RT-PCR、Northern Blot、芯片分析等 查看产品
·经典总RNA分离纯化方法·样本：血液、动物细胞、组织、植物组织等·适合从各种不同数量级的实验样本中分离纯化总RNA应用：RT-PCR、Northern Blot、芯片分析 查看产品
·1小时内即可完成miRNA的分离与纯化·柱分离技术快速高效、准确分离20-200nt的miRNA 片段·柱纯化技术确保获得高纯度的miRNA·应用：RT-PCR、Real-time RT-PCR、Northern Blot、 芯片分析 查看产品
·提供供两套裂解液体系，可以解决各种简单的植物组织、富含多糖多酚的植物组织、水果果肉、真菌等的 RNA 提取·无需使用酚氯仿、β-巯基乙醇等有毒试剂·无需耗时的醇类沉淀，可以快速提取总 RNA·过滤柱技术快速去除组织溶解物中的杂质 查看产品
·20-30分钟内即可完成凝胶中DNA的回收·最高可以达到85%的回收效率·核酸纯化柱无须平衡液处理，最大吸附30 μg DNA·溶液中所含的pH指示剂可直观地判断溶液中DNA与硅胶膜的最适宜结合条件·特别设计的纯化柱，可用25 μl微量洗脱体积洗脱DNA·应用：DNA测序、基因芯片分析、DNA连接与转化、限制性酶切、标记微注射、PCR、体外转录 查看产品
·8分钟内即可完成质粒DNA的制备，无须酚氯仿抽提·革命性的创新发明Buffer N8，2分钟内获得无漂浮沉淀的上清液·核酸纯化柱无须平衡液处理即可使用·特殊的添加中和指示剂设计，使初次使用者也可以获得最佳的质粒抽提效果 查看产品
·核酸纯化柱无须平衡液处理·特殊的添加中和指示剂设计，使初次使用者也可以获得最佳的质粒抽提效果·特别设计的Buffer W1 洗涤液，更彻底去除包括限制性内切酶在内的各种残留的蛋白，适用于包括野生型宿主菌在内的各种大肠杆菌菌株·应用：测序、限制性酶切、连接与转化、体外转录/翻译、强壮细胞转染 查看产品
本试剂盒含有全新高效的gDNase，3min高效去除gDNA，让基因组DNA在反转前高效去除；高效Quant逆转录酶，仅需15 min即可合成满足荧光定量实验的第一链cDNA，反转效率可高达90%。 查看产品
·1小时内完成植物总DNA的制备·特备适合DNA含量低的植物样本提取DNA·特别针对多糖、多酚含量高的植物或真菌样本设计 查看产品
经粪便保存液稳定的粪便样本可配套Simgen粪便DNA纯化试剂盒（配套粪便保存液使用产品，产品代号Cat.No.4111050）提取粪便DNA。粪便样本中的DNA经粪便保存液稳定后可在室温（15-25℃）保存6个月以上而免于降解，2-8℃或更低温度下可存放2年以上保持DNA不被降解。 查看产品