使用Simgen公司产品最新发表的论文(2021年9月-2022年10月)

作者:新景实验室
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(进入google学术搜索(用谷歌镜像地址http://scholar.hedasudi.com/可获得相同的结果),键入“simgen kit”即可获得大量文献)

通过谷歌学术搜索发现,2010年以来,就有大量科研人员使用Simgen产品进行科学研究并发表学术论文。不仅研究论文数量巨大,而且有一批高质量的注明使用Simgen产品的研究论文发表在Cell、Nature、Cell Stem Cell、Immunity、Developmental Cell、Cell Reports、Nature Medicine、Nature Immunology、Nature Chemical Biology、Nature Cell Biology、Nature Communications、PNAS、Blood、Gastroenterology、Nature Protocols、Plant Cell、Hepatology等高水平期刊上。

 

所有Simgen员工为此感到欢欣和鼓舞,感到骄傲和自豪!广大用户大量标注Simgen的研究论文的发表是对所有Simgen最大的支持和鼓励,最真切的认可和回报!

 

作为Simgen人,我们会再接再厉,不断开拓进取,为广大用户提供更多高质量的产品和服务,继续踏踏实实地为中国的生命科学事业添砖加瓦,以答谢广大用户对我们的支持和鼓励,对我们的认可和回报!

Simgen将永远和您一起为中国的生命科学事业的腾飞贡献自己的一份力量!!!

 

 

全血DNA小量试剂盒

1.Clinical characterization of NTCP deficiency in paediatric patients : A case‐control study based on?SLC10A1?genotyping analysis

LJ Deng, WX Ouyang, R Liu, M Deng, JW Qiu… - Liver …, 2021 - Wiley Online Library

… Peripheral blood of 2 mL was collected from each subject, and the genomic DNA was

extracted using a DNA extraction kit (Simgen, China) according to the manufacturer’s instructions. …

2. Mutational screening of AGRN, SLC39A5, SCO2, P4HA2, BSG, ZNF644, and CPSF1 in a Chinese cohort of 103 patients with nonsyndromic high myopia

YH Zheng, XB Cai, LQ Xia, FY Zhou, XR Wen… - Molecular …, 2021 - ncbi.nlm.nih.gov

… in peripheral blood by using the Blood DNA Mini Kit (Simgen, Hangzhou, China) [30]. …

The genomic DNA was sheared into 100 base pairs via the Exome Enrichment V5 Kit (Agilent …

3.Mitochondrial tRNA mutation and regulation of the adiponectin pathway in maternally inherited hypertension in chinese han

J Bai, Q Ma, Y Lan, Y Chen, S Ma, J Li, C Liu… - Frontiers in Cell and …, 2021 - frontiersin.org

… From each subject, 400 μL whole blood was treated according to the SimGEN Blood DNA

… The human ELISA kit was produced by Abcam. A standard sample or plasma, horseradish …

4.Novel mutations identified in the Chinese Han population with keratoconus by next-generation sequencing

B Chen, X Yu, X Zhang, H Yang, Y Cui… - Journal of …, 2022 - hindawi.com

… ese tubes were preserved at ?80C before genomic DNA was extracted with a Simgen DNA

Blood Mini Kit (Simgen, Hangzhou, China) according to the manufacturer’s instructions. All

5.Atheroprotective mechanism by which folic acid regulates monocyte subsets and function through DNA methylation

Y Xiang, B Liang, X Zhang, X Qiu… - Clinical …, 2022 - clinicalepigeneticsjournal …

… Genomic DNA was extracted using the phenol/chloroform method or a commercial DNA

extraction kit (3001050, Simgen, Hangzhou, China) based on the sample size and was …

6. Novel SOX2 mutation in autosomal dominant cataract-microcornea syndrome

ZB Lin, J Li, L Ye, HS Sun, A Yu, SH Chen, FF Li - BMC ophthalmology, 2022 - Springer

… A conventional DNA extraction kit (Simgen, Hangzhou, China) was used for genomic

DNA extraction. In addition, blood samples from another 200 healthy controls without of eye …


动物组织DNA试剂盒

1.The complete mitochondrial genome of the common strain black carp (Cyprinus carpio?var. baisenensis)

LJ Deng, WX Ouyang, R Liu, M Deng, JW Qiu… - Liver …, 2021 - Wiley Online Library

… Peripheral blood of 2 mL was collected from each subject, and the genomic DNA was

extracted using a DNA extraction kit (Simgen, China) according to the manufacturer’s instructions. …


植物/真菌DNA试剂盒

1.Abundant Genetic Diversity and Extensive Differentiation among Geographic Populations of the Citrus Pathogen?Diaporthe citri?in Southern China

T Xiong, Y Zeng, W Wang, P LiY GaiC Jiao, Z Zhu… - Journal of Fungi, 2021 - mdpi.com

… The scraped mycelia were lyophilized, and then the DNA was extracted using a SIMGEN

Fungi Kit (Simgen, Hangzhou, China) according to the manufacturer’s protocol. The extracted 

2.Genetic Diversity and Population Structure of?Phyllosticta citriasiana?in China

Y Zeng, T Xiong, B Liu, E Carstens, X Chen… - …, 2021 - Am Phytopath Society

… The mycelia were scraped from the plates and lyophilized before DNA extraction with a

SIMGEN Fungi Kit (Simgen, Hangzhou, China) according to the manufacturer’s protocol. The …

3.Botrytis cinerea?BcSSP2 protein is a late infection phase, cytotoxic effector

W Zhu, M Yu, R Xu, K Bi, S Yu, C Xiong… - Environmental …, 2022 - Wiley Online Library

… Total RNA of fungi and plant samples were isolated using RN03-RNApure Kit (Aidlab, …

cinerea strains was isolated using Fungal Genomic DNA Purification Kit (Simgen, Hangzhou…


植物DNA试剂盒

1. Citrus Huanglongbing detection based on polyphasic chlorophyll a fluorescence coupled with machine learning and model transfer in two citrus cultivars

H Weng, Y Liu, I Captoline, X Li, D Ye, R Wu - Computers and Electronics in …, 2021 - Elsevier

… it. The DNA was extracted using a Plant DNA kit (Simgen, Hangzhou, China). TaqMan

probes and primer pairs were synthesized according to the reported study by a

biotechnology company (Tsingke, Hangzhou, China). The …

2.An efficient transient gene expression system for protein subcellular localization assay and genome editing in citrus protoplasts

W Yang, J Ren, W Liu, D Liu, K Xie, F Zhang… - Horticultural Plant …, 2022 - Elsevier

… The genomic DNA was isolated from citrus protoplasts and the mutant shoots using plant

DNA Kit (SIMGEN, China). The genomic fragments containing the target sites were amplified by …

3.Microbial diversity on the surface of historical monuments in Lingyan Temple, Jinan, China

T Li, Y Cai, Q Ma - Microbial Ecology, 2022 - Springer

… Total genomic DNA was extracted with a Plant DNA Isolation Kit (Simgen, Hangzhou, China)

according to the manufacturer’s protocols. The DNA was subsequently used as a template …

4.The chloroplast genome of Salix floderusii and characterization of chloroplast regulatory elements.

W Ren, Z Jiang, M Zhang, L Kong, H Zhang… - Frontiers in plant …, 2022 - europepmc.org

… Total genomic DNA was extracted using the Plant DNA Extraction Kit (SIMGEN, Hangzhou,

China) according to the manufacturer’s instructions. DNA purity and integrity were verified via …


精液DNA纯化试剂盒

1.Complex genomic patterns of abasic sites in mammalian DNA revealed by a high-resolution SSiNGLe-AP method

Y Cai, H Cao, F Wang, Y Zhang, P Kapranov - Nature communications, 2022 - nature.com

… Genomic DNA from sperm cells was extracted using a Sperm DNA Purification Kit (Simgen,

4202050) following the manufacturer’s instructions. All DNA samples were eluted in …

Trizol

1.Vagus nerve stimulation protects enterocyte glycocalyx after hemorrhagic shock via the cholinergic anti-inflammatory pathway

J Wu, Y Yin, M Qin, K Li, F Liu, X Zhou… - Shock (Augusta …, 2021 - ncbi.nlm.nih.gov

… gut tissues using TRIzol reagent (Simgen, China). The same amount of total RNA was

reverse transcribed to cDNA using the ReverTra Ace qPCR RT kit (Simgen, China). Quantitative …

2.Intraperitoneal supplementation of iron alleviates dextran sodium sulfate-induced colitis by enhancing intestinal barrier function

L Liang, Q Xiong, J Kong, C Tian, L Miao… - Biomedicine & …, 2021 - Elsevier

… isolated using TRIzol reagent (Simgen, Hangzhou, China) … using a Whole Protein

Extraction kit (KeyGen BioTech, China) … an enhanced chemiluminescence detection kit (biosharp, …

3.Bioflocs attenuates lipopolysaccharide-induced inflammation, immunosuppression and oxidative stress in Channa argus

Z Yu, YY Zhao, N Jiang, AZ Zhang, MY Li - Fish & Shellfish Immunology, 2021 - Elsevier

… kits (Shanghai Biotechnology Co., Ltd., Shanghai, China). All measurement steps were

performed according to the relevant kit … extracted using TRIzol Reagent (simgen) according to the …、

4.A transcription factor DAF-5 functions in Haemonchus contortus development

W Di, F Li, L He, C Wang, C Zhou… - Parasites & …, 2021 - parasitesandvectors.biomedcentral …

… contortus using TRIzol reagent (Simgen, China). All RNA samples were treated with

DNase I to remove genomic DNA (gDNA), and then their integrity and yields were verified by …

5.H3K27ac chromatin acetylation and gene expression analysis reveal sex-and situs-related differences in developing chicken gonads

Y Jiang, Z Peng, Q Man, S Wang… - Biology of sex …, 2022 - bsd.biomedcentral.com

Total RNA was extracted using TRIzol reagent (Simgen) and cDNA was prepared using

PrimeScript RT reagent Kit with gDNA Eraser (Takara, RR047A). Analysis of mRNA expression …

6.中国南方一罕见迟发性脊椎骨骺发育不良大家系的表型和基因型的相关性研究

黄颖, 谢杰, 郑诗瑶, 唐佳, 郭奕斌 - 中山大学学报(医学科学版), 2022 - researchgate.net

...1.2.3 逆转录 PCR(RT-PCR)使 用 Trizol 试 剂(Simgen 试剂盒),从受试者新鲜外周血白细胞中提

取总 RNA,在 20 μL 体积内反向转录 1 000 ng RNA(Fermantas 试剂盒)...


超纯RNA试剂盒

1.Molecular Cloning and Functional Analysis of?GmLACS2-3?Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance

A Ayaz, H Huang, M Zheng, W Zaman, D Li… - International journal of …, 2021 - mdpi.com

… Triplicate biological samples of either soybean or Arabidopsis were used for the extraction

of total RNA using the Trizol total RNA extraction kit (SIMGEN, Beijing, China) according to …

2.A novel homozygous mutation in the PADI6 gene causes early embryo arrest

X Wang, H Zhu, Y He, J Zeng, J Zhao, Q Xia, L Wu… - Reproductive health, 2022 - Springer

… after transfection using the Total RNA Purification Kit (SimGen)Reverse transcription and

amplification were performed using the Evo M-MLV RT Mix Kit (Accurate Biotechnology), and …



全血总RNA试剂盒

1.Three Chinese pedigrees of A20 haploinsufficiency: clinical, cytokine and molecular characterization

Y Tian, B Wu, L Peng, J Wang, M Shen - Frontiers in Immunology, 2022 - ncbi.nlm.nih.gov

… Total RNA was extracted from frozen blood using an RNA isolation kit (Simgen, 5201005).

RNA was reverse transcribed using a 1st Strand cDNA Synthesis Kit (Takara, 6110). The …

2.Role of NINJ1 in Gout Flare and Potential as a Drug Target

H Zhang, J Gao, W Fang, Y Tang, X Fang… - Journal of …, 2022 - dovepress.com

… Total RNA was isolated from whole blood using a whole Blood RNA isolation kit (Simgen),

total RNA was isolated from cultured cells using Hipure total RNA mini kit (Magen). Isolated …

3.Transcriptome and DNA methylome analysis of peripheral blood samples reveals incomplete restoration and transposable element activation after 3-month recovery?…

Y Yin, X Liu, Q Tian, Y Fan, Z Ye, T Meng, G Wei…- medRxiv, 2022 - medrxiv.org

… Total RNA was isolated from 2.5ml blood using total RNA  isolation kit from SIMGEN. Isolated total RNA and remaining blood sample were subjected to mRNA sequencing and whole-…


 

病毒核酸纯化试剂盒


1.ANXA2 Facilitates Enterovirus 71 Infection by Interacting with 3D Polymerase and I4KB to Assist the Assembly of Replication Organelles

Q Zhang,S Li, P Lei, Z Li, F Chen, Q Chen, Y Wang… - Virologica Sinica, 2021 - Springer

… Three micrograms of RNA was reverse transcribed into cDNA using Toyobo RTkits with a …in each supernatant, viral nucleic acid isolation kits (Simgen, China) were used to extract the …

2.Discovery of Aryl Benzoyl Hydrazide Derivatives as Novel Potent Broad-Spectrum Inhibitors of Influenza A Virus RNA-Dependent RNA Polymerase (RdRp)

X Liu, J Liang, Y Yu,X Han, L Yu, F Chen… - Journal of Medicinal …, 2022 - ACS Publications… Supernatant virion RNA was extracted with the Viral Nucleic Acid Purification Kit (Simgen) according to the manufacturer’s instructions. Viral RNA was reverse transcribed into cDNA by …


粪便DNA纯化试剂盒

1. Nicotinic Acid Upregulates Intestinal Antimicrobial Peptides via Manipulating the Histone Acetylation Modification to Enhance the Resistance of Escherichia Coli?…

J Feng, D He, T Chen, W Cai, Y Chen, Y Xiong, Q Wu… - 2021 - researchsquare.com
… The contents in the colon of the piglets was aseptically collected, and the total DNA of the colonic contents was extracted using a DNA Kit (SimGEN, Hangzhou, China) according to the …

2.Effect of different dosages of sodium butyrate and niacin on growth, faecal microbiota and Vitamin B metabolism in weaned piglets

R Zhen, C Liu, C Wei, Y Luo, X Hu, G Liu…- Journal of Applied …, 2022 - Wiley Online Library 
… The feces of the piglets were aseptically collected, and the total DNA of the feces 111 was extracted using a DNA Kit (SimGEN, Hangzhou, China) according to the 112 instructions …

3.Effects of Niacin on Resistance to Enterotoxigenic Escherichia coli Infection in Weaned Piglets

R Zhen, J Feng, D He, Y Chen, T Chen, W Cai… - Frontiers in …, 2022 - ncbi.nlm.nih.gov
The contents in the colon of the piglets were aseptically collected, and the total DNA of the
colonic contents was extracted using a DNA Kit (SimGEN, Hangzhou, China) according to the …


质粒DNA小量试剂盒

1.Genome-wide characterization of C2H2 zinc-finger gene family provides insight into the mechanisms and evolution of the dehydration–rehydration responses in Physcomitrium and Arabidopsis                                                                               
Xuan Li, Cao, Jialing Li, Qinqin Niu, Yuanping Mo and  L Xiao - researchgate.net
cDNA was generated using HiScriptII 1st Strand cDNA Synthesis Kit (Vazyme, China). The gene… study were extracted and purified usin the Simgen Plasmid Extract Kit (Simgen, China). …


 

凝胶DNA回收试剂盒


1.Prenatal Detection of Novel Compound Heterozygous Splice Site Variants of the?KIAA0825?Gene in a Fetus with Postaxial Polydactyly Type A

Y Yao, S Deng, F Zhu- Genes, 2022 - mdpi.com
 Subsequently, products were excised from the gel, purified with a commercial kit (DNA Gel ExtractionKit, 2001250, SIMGEN, Hangzhou, China) and sequenced by Sanger sequencing. …

2.Clinical Performance of Nanopore Targeted Sequencing for Diagnosing Infectious Diseases

Y Fu, Q Chen, M Xiong, J Zhao, S Shen…- Microbiology …, 2022 - Am Soc Microbiol
… PCR products were analyzed by agarose gel electrophoresis and purified with a DNA gelextractionkit (Simgen). Sanger sequencing was performed on an ABI PRISM 3730 DNA …


 

cDNA第一链合成试剂盒

1.Comparative Transcriptome and sRNAome Analyses Reveal the Regulatory Mechanisms of Fruit Ripening in a Spontaneous Early-Ripening Navel Orange Mutant?…

L Mi, D Ma, S Lv, S Xu, B Zhong, T Peng, D Liu, Y Liu- Genes, 2022 - mdpi.com
… using the first strand cDNA synthesis kit (Simgen) according to the … using a SYBR Green PCR Mix kit (Simgen) with 10-μL … extracted using miRNA purification kits (Simgen, Hangzhou, …

2.Polymeric immunoglobulin receptor in dongtingking crucian carp (Carassius auratus indigentiaus): Molecular characterization and expression analysis in response to?     
 H Xia, P Yang, Z Chen, Y Zhang, L Liu… - Aquaculture …, 2022 - Wiley Online Library … 
...The total RNA was extracted using the animal tissue total RNA extraction kit (Simgen). RNA … Reverse transcription was performed usinga reverse transcription kit (Simgen)and the …

3.Integrated metabolomics and transcriptomics reveal flavonoids glycosylation difference in two Citrus peels

F Yu, X Xu, S Lin, T Peng,S Zeng - Scientia Horticulturae, 2022 - Elsevier                         
 
… Subsequently, 1 μg of total RNA was used for reverse transcription using SIMGEN cDNAfirst strand kit and gDNA Eraser was used to eliminate DNA (SIMGEN, Hangzhou). All cDNA …

4.甜橙SPL 基因家族的鉴定及其在成花诱导中的表达分析

杨杰, 陈蓉, 胡文娟, 吴巧玲, 陈健美…- 西北植物学报, 2022 -
使用植物总RNA 提取试剂盒(Simgen,中国杭州)提取样品总RNA,使用反转录试剂盒(Simgen,中国杭州)反转录合成cDNA.利用2×SYBR Green PCR Mix(Simgen,中国杭州)Light-Cycler480 …


动物组织总RNA提取试剂盒

1.Integrated transcriptome sequencing and RNA interference reveals molecular changes in?Diaphorina citri?after exposure to validamycin
HZ Yu, YX Xie, J Wang, Y Wang, YM Du… - Insect …, 2021 - Wiley Online Library… Total RNA was isolated from the control group (CK), V12, V24, and V36 using an animal tissue total RNA kit (Simgen, Hangzhou, China) following the manufacturer's protocol. In this …

2.Silencing of?Glycogen Synthase Kinase 3?Significantly Inhibits Chitin and Fatty Acid Metabolism in Asian Citrus Psyllid,?Diaphorina citri
JB Zhang, ZJ Lu, HZ Yu- International journal of molecular sciences, 2022 - mdpi.com
Thetotal RNA was isolated from D. citri in different tissues (midgut, leg, head, wing,integumentand fat body) and at different developmental stages (egg, 1st-, 2nd-, 3rd-,4th-and 5th-instar nymphs and adults) using the animal tissue total RNA kit (Simgen).

3.Polymeric immunoglobulin receptor in dongtingking crucian carp (Carassius auratus indigentiaus): Molecular characterization and expression analysis in response to?
H Xia, P Yang, Z Chen, Y Zhang, L Liu… - Aquaculture …, 2022 - Wiley Online Library
… The total RNA was extracted using the animal tissue total RNA extraction kit (Simgen). RNA… Reverse transcription was performed using a reverse transcription kit (Simgen)
and the …


快速质粒DNA小量试剂盒


1.An Early Diagnostic Clue for?COL18A1-?and?LAMA1-Associated Diseases: High Myopia With Alopecia Areata in the Cranial Midline

P Wang, X Jia, X Xiao, S Li, Y Long, M Liu…- Frontiers in Cell and …, 2021 - frontiersin.org… (HeLa) according to the manufacturer’s instruction (Rapid Plasmid Mini Kit, SIMGEN). The transfected cells were cultured for 48 h and then collected for transcript expression analysis. …

2.The identification of a novel splicing mutation in the DMD gene of a Chinese family
W Liu, X Shi, Y Li, F Qiao, Y Wu- Clinical Case Reports, 2021 - Wiley Online Library
… The positive colonies were used for extracting plasmid by Rapid Mini Plasmid Kit (Simgen),and plasmid was subsequently sequenced to confirm the inserts. The confirmed plasmids …

3.A Novel Mutation c. 3392G> T of COL2A1 Causes Spondyloepiphyseal Dysplasia Congenital by Affecting Pre-mRNA Splicing
L Fan, L Ji, Y Xu, G Shen, K Tang, Z Li, S Ye…- Frontiers in …, 2022 - ncbi.nlm.nih.gov… The wild-type and mutated amplified products were purified by a gel extractionkit and then … After plasmid amplification in DH5α competent cells and plasmid extraction (SIMGEN)
, the …


2×SYBR Green PCR Mix


1.
Comparative Transcriptome and sRNAome Analyses Reveal the Regulatory Mechanisms of Fruit Ripening in a Spontaneous Early-Ripening Navel Orange Mutant?…

L Mi, D Ma, S Lv, S Xu, B Zhong, T Peng, D Liu, Y Liu- Genes, 2022 - mdpi.com… using the first strand cDNA synthesis kit (Simgen) according to the … using a SYBR Green PCR Mixkit (Simgen) with 10-μL … extracted using miRNA purification kits (Simgen, Hangzhou, 

2.Conjugate of ibrutinib with a TLR7 agonist suppresses melanoma progression and enhances antitumor immunity
S Ren, X Wang, G Jin- International journal of biological sciences, 2022 - ncbi.nlm.nih.gov
… cDNA synthesis and real-time PCR were performed using a cDNA synthesis master mixkit and 2×SYBR Green PCR Mix (Simgen, Hangzhou, China).
 The following primers were used: c

3.甜橙SPL 基因家族的鉴定及其在成花诱导中的表达分析
杨杰, 陈蓉, 胡文娟, 吴巧玲, 陈健美…- 西北植物学报, 2022 -
… 使用植物总RNA 提取试剂盒(Simgen,中国杭州提取样品总RNA,使用反转录试剂盒(Simgen,国杭州)反转录合成cDNA.利用2×SYBR Green PCR Mix(Simgen,中国杭州)Light-Cycler480 …



粪便DNA保存液


1.Crosstalk between the gut microbiome and clinical response in locally advanced thoracic esophageal squamous cell carcinoma during neoadjuvant?…
L Xu, Y Qi, Y Jiang, Y Ji, Q Zhao, J Wu…- Annals of …, 2022 - ncbi.nlm.nih.gov
… The samples were dissolved in into fecal DNA preservation fluid (Simgen, 4103100,Hangzhou, China), transported to the laboratory, and stored at ?8 ℃ for subsequent processing. …


RNA样本保存液


1.Trigeminal Nerve Derived-Substance P Regulates Biological Characteristics of Limbal Stem Cells by Modulating the PI3K-AKT Signaling Pathway
P Xu, X Lin, X Dong, Y Liu, Z Wang, S Wang - Available at SSRN 4237970 - papers.ssrn.com
… Limbal tissues were quickly dissected and placed in RNA later reagent (Simgen, Hangzhou,China)for RNA isolation. Each sample contained three to five independent repeated mice …


植物总RNA 提取试剂盒


1.甜橙SPL 基因家族的鉴定及其在成花诱导中的表达分析

杨杰, 陈蓉, 胡文娟, 吴巧玲, 陈健美…- 西北植物学报, 2022 - xbzwxb.cnjournals.com
… 使用植物总RNA 提取试剂盒(Simgen,中国杭州) 提取样品总RNA,使用反转录试剂盒(Simgen,国杭州)反转录合成cDNA.利用2×SYBR Green PCR Mix(Simgen,中国杭州)Light-Cycler480 …


miRNA纯化试剂盒

1.Comparative Transcriptome and sRNAome Analyses Reveal the Regulatory Mechanisms of Fruit Ripening in a Spontaneous Early-Ripening Navel Orange Mutant?…

L Mi, D Ma, S Lv, S Xu, B Zhong, T Peng, D Liu, Y Liu- Genes, 2022 - mdpi.com… using the first strand cDNA synthesis kit (Simgen) according to the … using a SYBR Green PCR Mixkit (Simgen) with 10-μL … extracted using miRNA purification kits (Simgen, Hangzhou, 












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